Combining single-molecule imaging and single-channel electrophysiology.

10.1016/j.jmb.2014.07.007

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Simultaneous single-molecule fluorescence measurements of ion channel conformational change with single-channel electrophysiology.

Combining simultaneous single-molecule fluorescence measurements of ion channel conformational change with single-channel electrophysiology would enable a direct link between structure and function. Such methods would help us to create a truly molecular “movie” of how these important biomolecules work. Here we review past and recent progress toward this goal.